Optimization of Methylene Blue Encapsulated Liposomes: Comparison of MCD and PG Technologies of High Pressure Homogenisation Technique

Synopsis

Methylene Blue (MB) used for photodynamic therapy (PDT); is one of the promising anti-cancerous and photo-activatable antimicrobial drug. The limiting factor of MB therapy i.e. decreased cellular uptake can be prevented by encapsulation in liposomes. Various liposomal formulations loaded with MB were formulated and investigated for their osmolality, size, poly dispersity index (PDI), zeta potential, pH and drug entrapment efficiency. Formulations were processed at 100 & 200 MPa; one and two passes using MCD (1Y62, 2Y62, 2Z73, 3Z73) and PG technologies of high pressure homogenisation technique. Optimisation parameter was higher encapsulation efficiency and narrow size distributions. Formulations processed through MCD (1Y62) at 100MPa demonstrated relatively significant drug entrapment efficiency with narrow size distribution compared to other PCDs in MCD and PG technology. Study demonstrated that MCD technology yields more promising results than PG for designing of optimised MB liposomal formulations.